USFDA Nutritional Facts and Supplemental Facts Food Product Labels: Full label testing, nutrient data-base generated, analytical consultations
Testing Method, Recommended | ||
Nutritional Facts Panel Analysis (FDA & CFIA Compliance) |
Calories, Total | Calculated based on proximate. |
Fat, Total | AOAC 996.06, AACC GLC, AOCS GLC or equivalent: total fatty acids as triglycosides | |
Saturated Fatty Acidstrans Fatty Acids | AOAC 996.06, AACC GLC, AOCS GLC or equivalent | |
Cholesterol | AOAC 994.10, AACC GLC or equivalent | |
Carbohydrate, Total Carbohydrates | Calculated based on proximate: Total carbohydrates = (Serving size – ash – moisture – protein – fat) | |
Fibre, Total Dietary Fiber | AOAC 992.16 or 985.29, AACC or equivalent | |
Sugars | AOAC 980.13 or equivalent | |
Protein | AOAC 981.10 or 993.13 | |
Vitamin A | AOAC 992.04, AACC or equivalent | |
Vitamin C | AACC 86-10, or validated Fluorometric or HPLC, or equivalent | |
Sodium, Calcium, Iron | Validated ICP, AA, or equivalent | |
Food Nutritional Analysis (Additional Nutritional Parameters) |
Servings per Container | Claimed by the manufacturer |
Kilojoules, kJ per serving | Calculated based on proximate (see Calories). 1 Calorie = 4.186 kJ | |
Calories from Total FatCalories from Saturates + trans | Calculated based on a factor of 4 x g of the fatty acid, that’s determined by AOAC 996.06, AACC GLC, AOCS GLC or equivalent | |
Polyunsaturated Fatty AcidsOmega-6 PolyunsaturatesOmega-3 Polyunsaturates Monounsaturated Fatty Acids | AOAC 996.06, AACC GLC, AOCS GLC or validated GC methods | |
Soluble FiberInsoluble Fiber | AACC or equivalent | |
Sugar Alcohols, Polyols | Validated HPLC-RI or equivalent | |
Starch | AACC 76-13, AOAC or equivalent | |
Vitamin D (Vit D) | Validated HPLC-UV, or equivalent | |
Vitamin E (Vit E) | AACC 86-47 or equivalent | |
Vitamin K (Vit K) | Validated HPLC-UV, or equivalent | |
Thiamine (Thiamin, Vit B1) | AACC 86-80, or equivalent | |
Riboflavin (Vit B2) | AACC 86-70/72, or equivalent | |
Niacin | AACC 86-49/50A/51, or equivalent | |
Vitamin B6 (Vit B6) | AACC 86-31, or equivalent | |
Folate | AACC 86-47 or equivalent | |
Vitamin B12 (Vit B12) | AACC 86-40 or equivalent | |
Biotin | Validated HPLC-UV or immunoassays | |
Pantothenic Acid | Validated HPLC-UV or equivalent | |
Phosphorus, Magnesium, Zinc, Selenium, Copper, Manganese,Chromium, Molybdenum | Validated ICP or equivalent | |
Iodide (Iodine) | USP titration or equivalent | |
Chloride | Validated IC or equivalent |
Miscellaneous Food Tests | Test |
Acidity | Fat Acidity (General Method) |
Fat Acidity (Rapid Method for Small Grains) | |
Fat Acidity (Rapid Method for Corn) | |
Fat Acidity (Colorimetric Method) | |
Titratable Acidity | |
Neutralizing Value of Acid-Reacting Materials | |
Hydrogen-Ion Activity (pH) (Electrometric Method) | |
Acids | Phosphoric Acid (Qualitative Method) |
Phosphoric Acid (Quantitative Method) | |
Sulfuric Acid (Quantitative Method) | |
Acetic Butyric and Lactic Acids in Rye Flour | |
Benzoic Acid | |
Citric and Isocitric Acids | |
Tartaric Acid | |
Amino Acids:Aspartate, Glutamate, Asparagine, Glutamine, Glycine, Alanine, Valine, Leucine, Isoleucine, Histidine, Lysine, Arginine, Serine, Cysteine, Selenocysteine, Threonine, Methionine, Phenylalanine, Tyrosine, Tryptophan, Proline | Acid-Stable Amino Acids |
Sulfur Amino Acids | |
Tryptophan (Alkaline Hydrolysis) | |
Total Ash | Ash (Rapid Magnesium Acetate Method) |
Ash (Rapid 2-Hour 600° Method) | |
Ash in Cacao Products | |
Ash in Dry Milk Products | |
Ash in Farina and Semolina | |
Ash in Molasses Sugars and Syrups | |
Ash in Soy Flour | |
Ash in Starch | |
Ash in Yeast Foods | |
Biotechnology | Bt Cry1Ab-Modified Corn in Corn Flour (ELISA Method) |
StarLink Corn in Corn Flour and Corn Meal (ELISA Method) | |
ELISA Method for StarLink Corn in Corn Flour and Corn Meal | |
Color and Pigments | Pekar Color Test (Slick Test) |
Color of Pasta (Reflectance Colorimeter Method) | |
Agtron Color Test for Flour | |
Determination of Pigments | |
Ingredients | Egg Solids (Digitonin Cholesterol Method) |
Egg Solids in Pasta Products | |
Determination of Isoflavones in Soy and Selected Foods Containing Soy | |
Crude Fat | Crude Fat in Flour Bread and Baked Cereal Products Not Containing Fruit |
Crude Fat in Cocoa | |
Crude Fat in Baked Pet Food | |
Crude Fat in Dry Milk Products | |
Crude Fat in Egg Yolk by Acid Hydrolysis | |
Crude Fat in Grain and Stock Feeds | |
Crude Fat in Wheat Corn and Soy Flour Feeds and Mixed Feeds | |
Unsaponifiable Matter | |
Fiber | Total Dietary Fiber |
Total Dietary Fiber (Rapid Gravimetric Method) | |
Soluble Insoluble and Total Dietary Fiber in Foods and Food Products | |
Crude Fiber in Flours Feeds and Feedstuffs | |
Insoluble Dietary Fiber | |
Insoluble and Soluble Dietary Fiber in Oat Products (Enzymatic-Gravimetric Method) | |
beta-Glucan in Oat Fractions and Unsweetened Oat Cereals | |
beta-Glucan Content of Barley and Oats (Rapid Enzymatic Procedure) | |
Total Dietary Fiber (Uppsala Method) | |
Polydextrose in Foods by Ion Chromatography | |
Fructans in Foods and Food Products (Ion Exchange Chromatographic Method) | |
Measurement of Total Fructan in Foods by an Enzymatic/Spectrophotometric Method | |
Determination of trans-Galactooligosaccharides in Selected Food Products by Ion-Exchange Chromatographic Method | |
Resistant Starch in Starch Samples and Plant Materials | |
Total Dietary Fiber in Foods Containing Resistant Maltodextrin (Enzymatic-Gravimetric Method and Liquid Chromatography Determination) | |
Inorganic Constitutes | Chlorides in Ash as Sodium Chloride (Gravimetric Method) |
Chlorides in Ash as Sodium Chloride (Volumetric Method) | |
Chlorides in Yeast Foods—Quantitative Method | |
Iodine Determination | |
Determination of Minerals by Inductively Coupled Plasma Spectroscopy | |
Moisture | Air-Oven Methods, Air-Oven Method Drying at 135°, Volatile Matter in Fats and Shortenings, Volatile Matter in Soy Flours, Distillation with Toluene for Fats and Shortenings |
Starch | Starch (Glucoamylase Method with Subsequent Measurement of Glucose with Glucose Oxidase) |
Total Starch Assay Procedure (Megazyme Amyloglucosidase/alpha-Amylase Method) | |
Determination of Damaged Starch | |
Determination of Damaged Starch (Spectrophotometric Method) | |
Sugars | Determination of Simple Sugars in Cereal Products (HPLC Method) |
Determination of Saccharides by Liquid Chromatography | |
Determination of Glucose in Sugar Mixtures (Glucose Oxidase Method) | |
Determination of Sucrose | |
Determination of Reducing and Nonreducing Sugars | |
Vitamins | Vitamin A (Ultraviolet Absorption Method) |
Vitamin A in Enriched Flour | |
Vitamin A and Carotene | |
Analysis of Vitamins A and E by High-Performance Liquid Chromatography | |
Ascorbic Acid (Indophenol-Xylene Extraction Method) | |
Choline | |
Niacin in Enrichment Concentrates | |
Niacin and Niacinamide in Cereal Products | |
Riboflavin (Fluorometric Method) | |
Thiamine (Thiochrome Method) | |
B-Vitamins in Vitamin Concentrates by HPLC |
Food Contaminant Testing | Test |
Microorganisms | |
Aerobic Plate Count | |
Coliform (E. coli) | |
Clostridium perfringens Enumeration | |
Rope Spore Count | |
Salmonella Bacteria | |
Enumeration of Staphylococcus aureus | |
Enterococci | |
Thermophilic Spore Counts (Total Aerobic Flat-Sour H2S Non-H2S Anaerobic) | |
Thermophilic and Psychrotrophic Bacteria | |
Mold and Yeasts Counts | |
Ergot (Ether-Bicarbonate Method) | |
Smut (Microscopic Method) | |
Smut (Sedimentation Method) | |
Mycotoxins | Aflatoxin (Thin-Layer Chromatography Method) |
Aflatoxin (Liquid Chromatography Method) | |
Zearalenone (Thin-Layer Chromatography Method) | |
Zearalenone and alpha-Zearalenol in Corn (HPLC Method) | |
Identification of Aflatoxin on TLC Plates | |
Deoxynivalenol in Wheat (TLC Method) | |
Fumonisins B1 B2 and B3 in Corn (Liquid Chromatographic Method) | |
Pesticide Multi-Residue Screening | Please request parameter listing |
Nitrogen | Crude Protein (Improved Kjeldahl Method) |
Urea and Ammoniacal Nitrogen | |
Oxidizing Bleaching and Maturing Agents | Benzoyl Peroxide |
Quantitative Method for Bromates | |
Ammonium Persulfate | |
Azodicarbonamide in Premix | |
Special Properties of Fats Oils and Shortenings | Determination of Free Fatty Acids |
Determination of Peroxide Value (Acetic Acid-Chloroform Method) | |
Fatty Acid Composition by Gas Chromatography | |
Saturated Unsaturated Monounsaturated and Total Fats in Cereal Products by Acid Hydrolysis and Capillary Gas Chromatography | |
Iodine Value of Fats and Oils (Cyclohexane-Acetic Acid Method) | |
Acetone-Insoluble Lecithin | |
Melting Point (Capillary Method) | |
Monoglycerides and Free Glycerol | |
Saponification Value | |
Oil Stability Index | |
Smoke Flash and Fire Points |